What is important in preservation of samples with volatile organic compounds (VOCs), and is done by collecting sample in a completely filled container?

• A. Temperature control
• B. Nitrogen flushing
• C. Light exposure
• D. Zero headspace

Answer: (D)

VOCs are highly volatile, so preserving them means preventing loss to the surrounding air. Collecting the sample in a completely filled container removes any air gap, leaving no headspace for VOCs to evaporate into. This directly minimizes volatilization losses during collection, transport, and storage, keeping the sample’s composition intact until analysis. While temperature control or flushing with inert gas can further aid preservation, the act of having zero headspace is the most direct way to prevent VOC loss at the moment of collection.